Version: SPPIDER I SPPIDER II

Tradeoff between Sensitivity and Specificity 0.1 (Best recall) 0.2 0.3 0.4 0.5 (Balanced) 0.6 0.7 0.8 0.9 1.0 (Best precision)

Define interaction sites using RSA change threshold Absolute (Ų) Relative (%)

Tradeoff between Sensitivity and Specificity 0.1 (Best recall) 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 (Best precision)

Generate a new PDB file with B-factors replaced by prediction
encoded as Classification Regression and keep the rest chains (if any) in the file

Append solvent accessibility prediction results (from SABLE)

Print results in the CASP format

Present found interfaces as

As summary
Each interface separately

Append solvent accessibility prediction results (from SABLE)

Print results in the CASP format